https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30103 in vitro including conventional hemocytometer counting chamber, a luminescence-based assay that utilizes the change in the metabolic activity of viable cells as a measure of the relative number of cells, and a multi-mode cell imager that measures cell number using a counting algorithm. Each method presents its own advantages and disadvantages for the measurement of cell proliferation, including time, cost and high-throughput compatibility. This protocol demonstrates that each method could accurately measure cell proliferation over time, and was sensitive to detect growth at differing cellular densities. Additionally, measurement of cell proliferation using a cell imager was able to provide further information such as morphology, confluence and allowed for a continual monitoring of cell proliferation over time. In conclusion, each method is capable of measuring cell proliferation, but the chosen method is user-dependent.]]> Wed 17 Nov 2021 16:31:24 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52750 Tue 14 Nov 2023 11:55:15 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:44082 in vitro and orthotopic tumour growth in vivo. Dynamin inhibition reduced glioblastoma cell line viability and suppressed neurosphere formation and migration of GSCs. Tumour growth was reduced by CyDyn 4-36 treatment. Dynamin 2 inhibition therefore represents a novel approach for stem cell-directed Glioblastoma therapy.]]> Thu 06 Oct 2022 15:06:34 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27080 Sat 24 Mar 2018 07:40:44 AEDT ]]>